Easyroti 250 ml Phytoplankton Gel for Zooplankton Cultivation

Easyroti 250 ml - EASYREEFS

Description

Easyroti, for the cultivation of rotifers.

Two species of easy reefs® microalgae, Nannochloropsis (80%) and Tetraselmis (20%), are used for the cultivation of rotifers.

The dose depends on the density of rotifers reached.

The average consumption is 4 ml of Easyroti per liter of rotifer culture per day (harvesting 25% of the rotifers per day).

Available in two sizes: 250 ml, 1500 ml, and 3 L

Usage

Required materials3 cylindrical 2 L containers with gentle aeration, using a hollow rod. Initial inoculum of rotifers.

Necessary food Easyroti: Nannochloropsis (80%) and Tetraselmis (20%).

Culture cycle from the strain: 5 days.

To filter the rotifer, it is necessary to use a 50-micron mesh.

It consists of a parallel cultivation system in which, starting from day 6, continuous feeding is provided for the aquarium. One pot for "Cepa" and another pot for "Production".

Rotifer culture

The detailed dosage data below are under ideal conditions, assuming an ideal rotifer density. Sometimes, the amount of algae added may not meet the rotifer's demand. In the event of microalgae depletion (the water remains clear and yellowish), it will indicate that the dose was insufficient. When the rotifer lacks food for more than 4 hours, the number of eggs decreases, and consequently, the population growth rate also declines. It is essential that the rotifer always has food available.

The rotifer is very sensitive to changes in the physical and chemical parameters of the water. If the water quality deteriorates rapidly, the rotifer population will suffer. In case of excessive dirt in the tube, filter the rotifer and inoculate it in a clean tube, then add the necessary algae.

With experience, the intensity of the green color will indicate the amount of algae you need, and you may even develop your own protocol. There is no difference between using live algae and Easyroti as food.

A rod with a diameter of 5 mm is inserted into the culture tube with very gentle aeration (5-10 bubbles per second) to ensure a homogeneous mixing of the culture. Excessive turbulence causes the rotifer to lose its eggs, and they do not hatch.

It is recommended to distribute the feeding dose into two or more portions per day, but for time reasons, the rotifer is usually fed once a day.

DAY 1

Initial conditions: "Strain" culture with 500 ml. Number of rotifers: 400 rotifers/ml to 600 rotifers/ml and 4 ml of Easyroti Nannochloropsis (80%) + Tetraselmis (20%).

Final conditions: The culture will have a final volume of 1 L with algae.

Instructions for use: Disperse 4 ml of Easyroti in 0.5 L of seawater. Add the rotifer inoculum.

DAY 2

Initial conditions: 1 L of rotifer culture. It will be almost transparent and nearly free of algae. Estimated number of rotifers: 500 rot/ml.

Final conditions: The culture will have a final volume of 1.4 L with algae.

User manual: Disperse 4 ml of Easyroti in 0.5 L of seawater and add 0.4 L to the rotifer culture.

DAY 3

Initial conditions: 1.4 liters of rotifer culture. It will be almost transparent and almost algae-free. Estimated number of rotifers: 500-600 rot/ml.

Final conditions: The culture will have a final volume of 1.8 L with algae.

User manual: Disperse 8 ml of Easyroti in 0.5 L of seawater and add 0.4 L to the rotifer culture.

DAY 4

Initial conditions: 1.8 L of rotifer culture. It will be almost transparent and almost free of algae. Estimated number of rotifers: 600 rot/ml

Final conditions: The culture will have a final volume of 2 L with algae.

User manual: Disperse 6 ml of Easyroti in 0.25 L of seawater and add 0.2 L to the rotifer culture.

DAY 5

Initial conditions: 2 L of rotifer culture. It will be almost transparent and almost algae-free. Estimated number of rotifers: 700-800 rot/ml.

From this "Cepa" culture, a partial harvest of 0.5 L is carried out to start a new "Cepa" culture in another 2 L pot and begin a new cycle as on day 1.

The former crop "Cepa" will now be the "Production" culture. We will add Easyroti and increase it to 2 L. If the pot is too dirty, we need to transfer it to another clean one to continue the production culture.

Instructions: In the "Production" culture, we will add 8 ml of Easyroti previously dispersed in 0.5 L of seawater. In the "Cepa" culture, start a new cycle on the first day.

DAY 6

Initial conditions: Culture "Strain" as on day 2. The "Production" culture of 2 liters at 800 rot/ml will be almost transparent and nearly free of algae.

The "Production" culture will be used to harvest 0.5 L per day to be used as food in the aquarium (if the aquarium is not fed, the harvest should also be done to feed the rotifer with algae). Each time 0.5 L is harvested, 0.5 L must be added with 8 ml of Easyroti.

The "production" culture will be maintained until the pot is dirty (5-7 days).

When the "Cepa" culture reaches its 5th day, it will become a "Production" culture, and a new "Cepa" culture will be created in parallel, as explained for the 5th day.

We will empty the old "Production" cultivation and proceed with its cleaning and disinfection.